Biotangents introduce sequence variability into double-stranded DNA by using Short Sequence Randomisation™. This allows expression levels to be modulated through alterations in promoter and ribosome binding site sequences and allows target saturation mutagenesis in coding sequences. By the same process we can also uniquely tag or barcode DNA libraries of high sequence complexity.
Key features of our Short Sequence Randomisation™
Multiple locations – Regions of randomised DNA sequence can be introduced into one or several locations within the DNA construct
Single step – The insertion of a randomised DNA sequence into multiple DNA regions occurs in a single reaction
No amplification – A PCR-free approach preserves the uniqueness of each specific randomised sequence within a DNA library
Highly controlled randomisation – Regions of different lengths of randomised sequence are inserted at user-defined positions
High complexity sequences – Introduction of randomised sequences generates high complexity DNA libraries
Barcoding DNA constructs
As part of a collaboration with a leading synthetic biology company specialising in customised promoters, Biotangents developed a DNA barcoding approach utilising Short Sequence Randomisation™, allowing individual DNA constructs within complex libraries to be uniquely tagged and identified.